GSO ISO/TS 19006:2021

ISO/TS 19006:2016

Gulf Standard Current Edition · Approved on 01 July 2021

Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

GSO ISO/TS 19006:2021 Files

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GSO ISO/TS 19006:2021 Scope

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H₂DCFDA assay.

The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.